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. 2022 Sep 22;12:15831. doi: 10.1038/s41598-022-18262-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of diet on metabolic traits and adipose tissue characteristics. Mice were fed CD (white bars) and HFD (grey bars). Blood and tissue were collected from male (black dots) and female (white dots) mice at age 24 ± 1.5 weeks (n = 7–11/group) and analysed as described under Material and methods. (A) animal weight, (B) blood glucose, (C) serum insulin, (D) serum fatty acids and (E-J) relative mRNA levels of (E–F) adipokines, (G-H) chemokines and (I-J) inflammation markers of pancreatic (pWAT), epididymal (eWAT) and inguinal (iWAT) adipose tissue expressed as 2^−ΔCt relative to Rps13 mRNA (housekeeping gene) are presented as means ± SEM of n individual observations as indicated by the symbols in each plot. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 denote significant differences.