Fig. 3.

Dose-response inhibition curves of selected hits against 3CL^pro. (A) HEK293T cells were co-transfected with 3CL-flag and EYFP-linker-Rluc plasmid DNA at the ratio of 5:1. At 12 h post-transfection, the cells were re-seeded in 96-well plates (10⁴/well) and treated with serially diluted compounds. After 36 h, BRET ratio was measured. (B) CC₅₀ values in the cells were measured using CCK-8 kits. All data are expressed as mean ± SD of triplicate assays.