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. 2022 Sep 8;2(9):100289. doi: 10.1016/j.crmeth.2022.100289

Figure 4.

Figure 4

Detection of aggregated phospho-tau in the sarkosyl-insoluble fractions of Tau-P301L FBOs

(A) Total tau, phospho-tau, and tau isoform levels in the soluble fractions of 8- and 13-week 201B7 (control) FBOs overexpressing GFP or Tau-P301L. The white arrowheads indicate 4R-tau. The black arrowhead indicates 3R-tau. The data are expressed as the mean values ± SEM. For statistical analysis, two-way ANOVA was used (∗p < 0.05, ∗∗p < 0.004, ∗∗∗p < 0.0008) (n = 3). See also Table S2.

(B) Total tau and phospho-tau levels in the sarkosyl-insoluble fractions of 8- and 13-week 201B7 (control) FBOs overexpressing GFP or Tau-P301L. The arrowheads indicate 4R-tau. The data are expressed as the mean values ± SEM. For statistical analysis, two-way ANOVA was used (∗p = 0.0436) (n = 3). See also Table S2.

(C) Tau inclusions in 13-week 201B7 (control) FBOs overexpressing GFP or Tau-P301L, and the brains of a control and a patient with AD were stained with thioflavin S and AT8. Images are representative of 201B7 (control) FBOs overexpressing GFP or Tau-P301L that were imaged with these markers (n = 3). Scale bar, 10 μm.