REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
B2M-APC	Biolegend	316312; RRID:AB_10641281
CD90-APC	Biolegend	328113; RRID:AB_893440
β-Catenin	BD	610153; RRID:AB_397554
Goat anti-mouse IgG, Alexa Fluor 647	Thermo Fisher Scientific	A-21235; RRID:AB_2535804
Recombinant DNA
PB-CRISPR	Addgene	160047
pCE-mp53DD	Addgene	41856
pGuide (for DNA plasmid gRNA delivery)	Addgene	64711
XLoneV3-ABE8e	This paper	Pending submission
modRNAc0-Cas9	This paper	Addgene 170180
modRNAc0-Cas9-2A-GFP	This paper	Addgene 170181
modRNAc0-Cas9-2A-Puro	This paper	Addgene 172855
modRNAc0-p53DD	This paper	Addgene 176902
modRNAc0-ABE8e	This paper	Addgene 178177
Chemicals, peptides, and recombinant proteins
iMatrix-511	Iwai North America Inc	N-892021
mTeSR1	StemCell Technologies	85850
Accutase	Innovative Cell Technologies	AT104500
DMEM	Thermo Fisher Scientific	11965118
Y-27632	Selleck Chemicals	146986-50-7
N1-methyl-pseudo-UTP	TriLink Biotechnologies	N-1081
Anti-Reverse Cap Analog (ARCA)	TriLink Biotechnologies	N-7003
CleanCap AG	TriLink Biotechnologies	N-7113
Lipofectamine Stem Transfection Reagent	Thermo Fisher Scientific	STEM00015
Opti-MEM Reduced Serum Medium	Thermo Fisher Scientific	51985034
Doxycycline	Sigma-Aldrich	D9891
PBS	Thermo Fisher Scientific	14190250
Bovine Serum Albumin	VWR	10842-692
GoTaq G2 Hot Start Master Mix	Promega	M7422
LB Broth medium	Thermo Fisher Scientific	10855001
Puromycin	Thermo Fisher Scientific	A1113803
Critical commercial assays
ZymoClean Gel DNA Recovery kit	Zymo Research	D4001
In-Fusion HD Cloning Plus CE kit	Takara Bio	638916
DNA Clean & Concentrator-5	Zymo Research	D4004
MEGAscript T7 Transcription kit	Thermo Fisher Scientific	AM1334
MEGAclear transcription clean-up kit	Thermo Fisher Scientific	AM1908
EnGen sgRNA Synthesis kit, S. pyogenes	NEB	E3322
Zymo Quick DNA Miniprep Plus kit	Zymo Research	D4068
TOPO TA Cloning Kit for Sequencing	Thermo Fisher Scientific	45-003-0
Zyppy Plasmid Miniprep Kit	Zymo Research	D4020
Experimental models: Cell lines
Human: H9 hESCs	WiCell	WB0299
Human: H1 OCT4-GFP hESCs	WiCell	MCB-01
Human: IMR90C4 iPSCs	WiCell	WB65317
Software and algorithms
FlowJo	http://www.flowjo.com/	N/A
Oligonucleotides
For gene cloning, sequencing editing sites, and gRNA sequences.	This paper	See Table S1