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. 2022 Sep 22;12:124. doi: 10.1186/s13568-022-01464-0

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Radial caseinolytic assay for SAK activity measurement on 5% skim milk-agar plate. a shows the expression of SAK in the dicistronic SILEX system at optimized conditions for three clones (wells 1, 2, and 3 are replicates). b shows the positive and negative control for SAK activity [well 2: commercial SAK with plasminogen (As the positive control), and well 3: Commercial SAK without plasminogen (As the negative control)]