Figure 3.

Antimicrobial activity of recombinant Pru p 7 (blue circles) in comparison with snakin-2 (red triangles), a cysteine-rich protein from potato reported to exhibit antimicrobial properties and thus considered as a positive control in the experiment. Various concentrations of recombinant Pru p 7 and snakin-2 were mixed with cell suspension in potassium phosphate buffer (10 mM, pH 6.0) and incubated at 37°C for bacteria (E. coli and S. aureus) and 30°C for fungi (C. parapsilosis) for 1 h. After incubation, the reaction mixture was diluted and plated on agar plates. Tryptic soy broth plates were used for bacteria and Sabouraud agar plates for fungi. After incubation, colonies were counted for calculating the survival rates. A representative experiment is shown and each point was performed in simplicate.