Table 3.

Concentrations of HNE Used in Previous Articles for HepG2 Cells

Plate	Cell concentration	HNE concentration	Incubation time	Reference
25-cm2 plate	2×105 cells/cm2	2–50 μM	24 hours	Muzio et al.42
96-well	–	5–20 μM	1, 2, 4, 8, 24 hours	Gallagher et al.43
6-well	5 × 106 cells/well	20–100 μM	15, 30, 60 minutes	Stewart et al.44
–	1 × 107 cells/well	100 μM	1 hour	Shearn et al.45
–	1 × 106–1 × 107 cells/well	100 μM	1 hour	Shearn et al.46
12-well	5 × 105 cells/well	12.5–100 μM	16 hours	Shearn et al.47
–	–	40 μM	24 hours	Chaudhary et al.48
96-well	2 × 103 cells/well	70 μM	24 hours	Zhang et al.49

HNE, 4-Hydroxy-2-nonenal.

Dash indicates that it was not described.