Supplementary Table 1A.

Solutions

Solutions	Reagents	Final concentration
EGTA perfusion solution	NaCl	136.89 mM
	KCl	5.37 mM
	NaH2PO4	0.64 mM
	Na2HPO4	0.85 mM
	HEPES	9.99 mM
	NaHCO3	4.17 mM
	EGTA	0.50 mM
	Glucose	5.00 mM
Enzyme buffer	NaCl	136.89 mM
	KCl	5.37 mM
	NaH2PO4	0.64 mM
	Na2HPO4	0.85 mM
	HEPES	9.99 mM
	NaHCO3	4.17 mM
	CaCl2·2H2O	3.81 mM
Gey’s balanced salt solution B, GBSS/B	NaCl	136.89 mM
	KCl	4.96 mM
	MgCl2·6H2O	1.03 mM
	MgSO4·7H2O	0.28 mM
	Na2HPO4	0.42 mM
	KH2PO4	0.22 mM
	Glucose	5.50 mM
	NaHCO3	2.70 mM
	CaCl2·2H2O	1.53 mM
Gey’s balanced salt solution A, GBSS/A	KCl	4.96 mM
	MgCl2·6H2O	1.03 mM
	MgSO4·7H2O	0.28 mM
	Na2HPO4	0.42 mM
	KH2PO4	0.22 mM
	Glucose	5.50 mM
	NaHCO3	2.70 mM
	CaCl2·2H2O	1.53 mM
Pronase E perfusion solution	Pronase E in enzyme buffer	1 mg/mL
Collagenase perfusion solution	Collagenase in Enzyme buffer	1.33 mg/mL
In vitro digestion solution	Pronase E	0.556 mg/mL
	Collagenase	0.556 mg/mL
	DNase I in Enzyme buffer	0.02 mg/mL