Figure 2.

Functional studies of patient 1. (A) Detection of natural immune responses in peripheral blood mononuclear cells (PBMCs) separated from the patient and healthy controls (n = 3). Pro‐inflammatory cytokines production of TNF‐α, IL‐6, and IL‐1β in response to 24‐h stimulations with LPS, TDB, β‐glucan, and P. expanda conidia (HK or viable resting) was detected in the supernatants by commercial ELISA kits (B) Detection of acquired immune responses in PBMCs separated from the patient and healthy controls. Cytokine production of IL‐17A, IL-22 in response to LPS, TDB, β‐glucan, and P. expanda conidia (HK) stimulations were measured in the cell culture supernatants after 6 days using commercial ELISA kits (n = 3). The proportions of Th17 (IL‐17+CD4+) Th22 (IL‐22+IL‐17‐CD4+) cells in the total number of CD4+ T cells were counted by flow cytometry after 6‐day stimulation (n = 6). LPS, lipopolysaccharide; TDB, Trehalose dibehenate; P1, patient1; Th, T helper type.