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. 2022 Sep 8;24(5):667. doi: 10.3892/etm.2022.11603

Figure 2.

Figure 2

Asiaticoside effectively protects HaCat cells from cellular senescence induced by UV exposure. (A) Morphological results of HaCat cells after subjecting a treatment of UV and incubation of asiaticoside with various concentration for 24 h (scale bar=100 µm; magnification x100, x200 and x400 from top to bottom). (B) Detection of β-galactosidase activity in HaCat cells after UV exposure and incubation of asiaticoside with various concentration (scale bar=200 µm). (C) Corresponding analytic result of optical data to activity of β-galactosidase. (D) Flow cytometry analysis of fluorescence intensity of HaCat cells after treatment with UV and asiaticoside in various concentrations. (E) Corresponding analytic result of fluorescence intensity to ROS positive cells. (F) Analysis of SOD activity of HaCat cells after treatment with UV and asiaticoside in various concentrations. *significance of comparison between UV with WT group; #significance of comparison between treatment of asiaticoside in various doses with UV group. ***P<0.001, ****P<0.0001, ###P<0.001 and ####P<0.0001. UV, ultraviolet; WT, wildtype; Asi, asiaticoside; ROS, reactive oxygen species; SOD, superoxide dismutase.