TABLE 2.
Plasmids used
| Plasmid | Vector | Relevant characteristics | Source or reference(s) |
|---|---|---|---|
| pCHAP231 | pBR322 | pulS pulAB pulC to pulO (Apr) | 12 |
| pCHAP1219 | pBR322 | As pCHAP231 but pulS::Tn5 (Apr) | 12, 26 |
| pCHAP1325 | pBR322 | As pCHAP231 but ΔpulK::kan-2 (Apr) | 26 |
| pCHAP580 | pSU19 | lacZp-pulS (Cmr) | 11, 26 |
| pCHAP5506 | pSU19 | As pCHAP580 but pulS codes for protein with C-terminal His8 extension | This study |
| pCHAP3094 | pSU19 | As pCHAP580 but with Cys36Ser substitution in PulS (Cys1−) (Cmr) | This study |
| pCHAP3095 | pSU19 | As pCHAP580 but with Cys90Ser substitution in PulS (Cys2−) (Cmr) | This study |
| pCHAP3093 | pSU19 | As pCHAP580 but with Cys36Ser and Cys90Ser substitutions in PulS (Cys1− Cys2−) (Cmr) | This study |
| pCHAP1371 | pHSG576 | As pCHAP580 (Cmr) | This study |
| pCHAP1377 | pHSG576 | As pCHAP3095 (Cmr) | This study |
| pCHAP1270 | pSU18 | pulK (Cmr) | 26 |
| pCHAP1271 | pSU18 | (lacZ′-′pulK) (Cmr) | 26 |
| pCHAP3129 | pSU18 | As pCHAP1270 but with Cys96Ser substitution in PulK (Cmr) | This study |
| pCHAP1374 | pHSG575 | As pCHAP1270 (Cmr) | This study |
| pCHAP1375 | pHSG575 | As pCHAP3129 (Cmr) | This study |
| pCHAP4260a | pUC18 | lacZp-(pelB′-′pulA) (Apr) | O. Francetic |
| pCHAP1367 | pUC18 | As pCHAP4260 but with pulK gene from pCHAP1270 cloned behind (pelB′-′pulA) (Apr) | This study |
| pCHAP1368 | pUC18 | As pCHAP4260 but with pulS gene from pCHAP580 cloned behind (pelB′-′pulA) (Apr) | This study |
| pCHAP163 | pHSG576 | lacZp-pulG | 27 |
| pCHAP3100 | pSU18 | lacZp-ppdD (Cmr) | 31 |
| pCHAP576 | pBGS19 | lacZp-pulO | 26 |
| pCHAP710 | pACYC184 | pulS, pulC to pulO (Cmr) | 17 |
a
pCHAP4260 will be described in greater detail elsewhere (O. Francetic and A. P. Pugsley, unpublished data).