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Complementation of pulS::Tn5 carried by pCHAP1219 by pulS alleles carried by compatible plasmidsa
| Complementing plasmid | pulS mutation | IPTG induction | Pullulanase secretion (%) |
|---|---|---|---|
| pCHAP580 | − | 80–100 | |
| pCHAP1371 | − | 80–100 | |
| pCHAP3094 | Cys1− | + | 0–10 |
| pCHAP3095 | Cys2− | − | 40–70 |
| + | 80–100 | ||
| pCHAP1377 | Cys2− | − | 0–10 |
| + | 10–20 | ||
| pCHAP3093 | Cys1− Cys2− | + | 0–10 |
The host strain was PAP7460.
