TABLE 4.

Complementation of pulK mutation carried by pCHAP1325 by pulK alleles carried by compatible plasmids^a

Complementing plasmid	pulK mutation	IPTG induction	Pullulanase secretion (%)
pCHAP1270		−	80–100
pCHAP1374		−	80–100
pCHAP1271	lacZ′-′pulK	−	80–100
pCHAP3129	Cys1−	−	80–100
pCHAP1375	Cys1−	−	0–10
		+	30–40

^aThe host strain was PAP7460.