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. 2022 Sep 9;13:946584. doi: 10.3389/fpls.2022.946584

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Copyright © 2022 Maywald, Mang, Pahls, Neumann, Ludewig and Francioli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Box plots of the bacterial observed richness. (A) Principal coordinates analysis (PCoA) based on Bray–Curtis dissimilarity. (B) Colors: without nitrogen, black; calcium nitrate, orange; ammonium sulfate, green; calcium cyanamide, blue. Effects of N fertilization were tested with PERMANOVA (based on Hellinger transformed data and the Bray–Curtis distance measure). (C) Mean relative abundance of the main classes of the bacterial communities detected. The same lowercase letters indicate no significant differences at 0.05 probability level according to Tukey multiple comparison test.