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. 2022 Sep 9;12:966361. doi: 10.3389/fcimb.2022.966361

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Copyright © 2022 Li, Methé, Fitch, Gentry, Kessinger, Patel, Petraglia, Swamy and Morris

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Variables and Models. The left panel (A) summarizes the groups of variables that were utilized in the analyses. Pre-pandemic (PP) (blue) and Early Pandemic (EP) (beige) variables include the taxonomic profiles from sequencing microbiota samples, BMIs interpolated based on the sample collection dates, and timespans relative to March 15, 2020. The questionnaire responses were only collected during the early pandemic. The top right panel (B) illustrates the early pandemic cross-sectional model. Here, only the EP variables: timespan, BMI, and questionnaire were utilized to build a model to predict the EP stool or saliva microbiota. The lower right panel (C) represents the variables included in the paired and cluster transition models. Both the PP and EP timespans, as well as questionnaire responses were included in the models. The BMI and microbiota profiles were included in the model as their relative changes which could be calculated per subject.