Table 2.
Polymorphic sites in the sequence comparison of the nuclear rDNA complete intergenic region and in the ITS-1 and ITS-2 between haplotypes of “genetically pure” Fasciola hepatica from livestock in Europe and the Americas, haplotypes of “genetically pure” F. gigantica from livestock in African countries, and the haplotype of F. nyanzae from hippopotamuses in the nature reserves in South Africa.
| Fasciola spp. and combined haplotypes |
Polymorphic sites
Intergenic region (ITS-1, 5.8S, ITS-2) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Positions | 24 | 114 | 208 | 286 | 306 | 821 | 834 | 860 | 866 | 874 | 917 | 924 |
| Polymorphic sites ITS-1 | Polymorphic sites ITS-2 | |||||||||||
| Positions | 24 | 114 | 208 | 286 | 306 | 234 | 248 | 273 | 279 | 288 | 330 | 337 |
| F. hepatica 1A | C | A | C | T | C | T | A | C | C | C | T | G |
| F. hepatica 2A | C | A | C | T | C | T | A | C | C | T | T | G |
| F. hepatica 1/2A* | C | A | C | T | C | T | A | C | C | C/T | T | G |
| F. gigantica 1A | T | T | T | A | T | C | A | T | T | C | - | A |
| F. gigantica 2A | T | T | T | A | T | C | C | T | T | C | - | A |
| F. gigantica 1/2A** | T | T | T | A | T | C | C/A | T | T | C | - | A |
| F. nyanzae 1A | T | A | T | T | C | T | A | T | C | C | T | A |
*
Heterozygotic in position 874/288 not differentiating between F. hepatica and F. gigantica.
**
Heterozygotic in position 834/248 not differentiating between F. hepatica and F. gigantica- also designed as H3A in Chougar et al. (27).