Table 5.

Estimates of evolutionary divergences over sequence pairs between species groups in the complete sequences of the nuclear rDNA and mtDNA markers.

(A) rDNA ITS-1
F. nyanzae
F. gigantica	3.0
F. hepatica	2.0	4.4
Fs. jacksoni	17.0	17.4	15.0
Fs. magna	26.5	25.9	24.5	25.5
(B) rDNA ITS-2
F. nyanzae
F. gigantica	2.3
F. hepatica	2.3	4.4
Fs. jacksoni	32.0	30.3	34.3
Fs. magna	37.0	37.3	39.3	29.0
(C) Complete intergenic region
F. nyanzae
F. gigantica	6.4
F. hepatica	5.3	8.9
Fs. Jacksoni	50.0	47.8	49.3
Fs. magna	63.5	64.3	64.8	55.5
(D) mtDNA cox1 gene
F. nyanzae
F. gigantica	119.9
F. hepatica	140.9	130.6
Fs. Jacksoni	215.6	199.8	212.0
Fs. magna	198.8	192.9	205.4	187.7
(E) mtDNA nad1 gene
F. nyanzae
F. gigantica	80.8
F. hepatica	77.2	75.6
Fs. jacksoni	140.9	132.0	133.6
Fs. magna	136.4	133.4	136.6	106.0

The number of base differences (ts + tv) per sequence from averaging over all sequence pairs between groups are shown. All ambiguous positions were removed for each sequence pair. Evolutionary analyses conducted in MEGA X.