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. 2022 Sep 9;9:990872. doi: 10.3389/fvets.2022.990872

Table 6.

Nucleotide and amino acid differences found in the complete mtDNA cox1 sequence of Fasciola nyanzae from South Africa and other F. nyanzae isolate fragments from Zimbabwe.

F. nyanzae haplotype (H) or isolate fragment (I) Locality* Country Host** GenBank Acc. No. cox 1 nucleotide sequence COXI amino acid sequence
Variable positions Long (bp) AT (%) Variable positions Length (aa)
11 1111111111 113443
1222233344 4445666666 8999999900 0000013445 6373499
9001300400 4492014569 0256669900 0111475992 8547089
5139116538 1450785199 7783690325 9148130364
H cox1-a K South Africa H.amph ON661094 CGTCTTGATA CTCCCTTGAT CGCCGACCGG AAGATGCATA 1,533 60.60 MWHMIHI 510
H cox1-b K South Africa H.amph ON661095 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C . . 1,533 60.53 . . . . . P . 510
H cox1-c S South Africa H.amph ON661096 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . AT . AG 1,533 60.67 . . . . . . N 510
H cox1-d S South Africa H.amph ON661097 . . . . C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . AG 1,533 60.47 . . . . . . N 510
H cox1-e S South Africa H.amph ON661098 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . AG 1,533 60.53 . . . . . . N 510
H cox1-f S South Africa H.amph ON661099 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A . . AG 1,533 60.60 . . . . . . N 510
H cox1-g K South Africa H.amph ON661100 . . . . . . . . . . . . . T . . . . . . . . . . . . . . . . . . . . . AT . AG 1,533 60.73 . . Y . . . N 510
I S8b LNA 1 Kariba Zimbabwe R.nat MT909550 TA . . . . . . C . . . . . TA- - - - - - - - - - - - - - - - - - - - - - - - 428 59.35 . R . - - - - 142
I KAR S3 RAD 10 Kariba Zimbabwe R.pli MT909549 TAA . . . . C . . . . . . T . CCG- - - - - - - - - - - - - - - - - - - - - 482 58.92 K . . - - - - 160
I 483 Kariba Zimbabwe R.nat MT909548 TA . . . . . . . . TC . . T- - - - - - - - - - - - - - - - - - - - - - - - - 425 59.29 . . . - - - - 141
I 480 Kariba Zimbabwe R.nat MT909546 TA . . . . AG . G T . T . . - - - - - - - - - - - - - - - - - - - - - - - - - 424 59.43 . . . - - - - 141
I 32 Kariba Zimbabwe P.col MT909545 TA . T . . . . . . . . . . T - - - - - - - - - - - - - - - - - - - - - - - - - 425 59.53 . . . - - - - 141
I 2 Kariba Zimbabwe H.amph MT909543 - - - T . . . . . . . . . . T . . TG TA TGTTTTAA GTTGG- - - - - 862 60.90 ? . . VV - 287
I 1 Kariba Zimbabwe H.amph MT909542 - - . . . C . . . . . . . . . . . . GC . . . T . . . . . . . . . - - - - - - - 815 59.39 ? . . . - - - 271

Positions, numbers (to be read in vertical) refer to variable positions obtained in the 1,533-bp-long alignment made with MEGA X; . = identical; shaded positions with a gap = not sequenced.

*

K, = Klaserie Private Nature Reserve; S, = Sabi Sand Nature Reserve.

**

H.amph, Hippopotamus amphibius; R.nat, Radix natalensis; R.pli, Radix plicatula; P.col, Pseudosuccinea columella.