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. 2022 Sep 9;9:980690. doi: 10.3389/fvets.2022.980690

Figure 1.

Figure 1

Effect of AOAE on the expression of TLR4/NF-κB pathway related genes in liver of broilers challenged with LPS (1) TLR4, toll like receptor 4; MyD88, myeloid differentiation factor 88; TRAF6, tumor necrosis factor receptor associated factor 6; NF-κB p65, nuclear factor kappa B p65; NF-κB p50, nuclear factor kappa B p50; IκBα, inhibitor of NF-κB alpha; IL-1β, interleukin 1 beta; IL-6, interleukin 6; PPARγ, peroxisome proliferator-activated receptor γ. (2) AOAE, Artemisia ordosica alcohol extract; CON, non-challenged broilers fed a basal diet; LPS, lipopolysaccharide-challenged broilers fed a basal diet; AOAE-L, lipopolysaccharide-challenged broilers fed a basal diet supplemented with 500 mg/kg AOAE; AOAE-M, lipopolysaccharide-challenged broilers fed a basal diet supplemented with 750 mg/kg AOAE; AOAE-H, lipopolysaccharide-challenged broilers fed a basal diet supplemented with 1,000 mg/kg AOAE. (3) The gene expression for β-actin was used as a housekeeping gene. The relative expression levels from the control group were used as reference values. Each value is shown as mean ± SD (n = 6). Bars that do not share the same letters (a, b, c, and d) are significantly different (P < 0.05) from each other.