Table 7.
Effect of AOAE on splenic cytokines and immunoglobulins in broilers challenged with LPS.
| Items | Treatments | P-value | ||||
|---|---|---|---|---|---|---|
| CON | LPS | AOAE-L | AOAE-M | AOAE-H | ||
| 28 d | ||||||
| IL-1β, pg/mg prot. | 13.09 ± 2.19c | 33.32 ± 3.77a | 26.19 ± 2.33b | 23.23 ± 3.49b | 14.73 ± 2.32c | <0.0001 |
| IL-2, pg/mg prot. | 6.40 ± 0.97 | 8.06 ± 0.92 | 7.75 ± 1.31 | 7.59 ± 1.44 | 7.33 ± 1.57 | 0.2354 |
| IL-4, pg/mg prot. | 4.01 ± 0.59 | 3.93 ± 0.57 | 4.56 ± 0.76 | 4.48 ± 0.75 | 4.51 ± 0.52 | 0.2928 |
| IL-6, pg/mg prot. | 0.59 ± 0.11c | 2.71 ± 0.49a | 2.75 ± 0.37a | 2.70 ± 0.18a | 0.97 ± 0.17b | <0.0001 |
| IgA, μg/mg prot. | 9.13 ± 1.09 | 8.22 ± 0.74 | 8.70 ± 1.39 | 9.11 ± 1.52 | 8.78 ± 0.47 | 0.6152 |
| IgG, μg/mg prot. | 84.32 ± 10.57a | 65.68 ± 9.61b | 60.18 ± 11.25b | 63.03 ± 11.32b | 87.41 ± 16.79a | 0.0009 |
| IgM, μg/mg prot. | 27.78 ± 1.86a | 16.93 ± 1.96c | 22.00 ± 1.79b | 26.33 ± 5.30a | 26.71 ± 3.71a | <0.0001 |
| 42 d | ||||||
| IL-1β, pg/mg prot. | 11.36 ± 1.30b | 15.27 ± 3.13a | 12.17 ± 2.16b | 11.47 ± 1.69b | 12.55 ± 1.65b | 0.0214 |
| IL-2, pg/mg prot. | 8.60 ± 0.85 | 7.86 ± 1.65 | 8.00 ± 1.19 | 7.76 ± 1.17 | 7.85 ± 1.81 | 0.8300 |
| IL-4, pg/mg prot. | 4.12 ± 0.47 | 3.92 ± 0.72 | 3.66 ± 0.58 | 4.27 ± 0.59 | 4.54 ± 0.91 | 0.2318 |
| IL-6, pg/mg prot. | 0.70 ± 0.09 | 0.71 ± 0.11 | 0.73 ± 0.06 | 0.69 ± 0.10 | 0.71 ± 0.10 | 0.9742 |
| IgA, μg/mg prot. | 6.96 ± 1.13 | 7.35 ± 0.72 | 6.85 ± 1.10 | 6.73 ± 0.97 | 7.56 ± 1.50 | 0.6689 |
| IgG, μg/mg prot. | 72.84 ± 4.72ab | 64.50 ± 7.08b | 66.13 ± 9.40b | 73.13 ± 7.98ab | 83.72 ± 16.62a | 0.0224 |
| IgM, μg/mg prot. | 27.26 ± 4.97a | 19.14 ± 2.86c | 21.23 ± 3.60bc | 22.60 ± 3.27bc | 24.35 ± 3.14ab | 0.0087 |
1) IL-1β, interleukin 1 beta; IL-2, interleukin 2; IL-4, interleukin 4; IL-6, interleukin 6; IgA, immunoglobulin A; IgG, immunoglobulin G; IgM, immunoglobulin M.
2) AOAE, Artemisia ordosica alcohol extract; CON, non-challenged broilers fed a basal diet; LPS, lipopolysaccharide-challenged broilers fed a basal diet; AOAE-L, lipopolysaccharide-challenged broilers fed a basal diet supplemented with 500 mg/kg AOAE; AOAE-M, lipopolysaccharide-challenged broilers fed a basal diet supplemented with 750 mg/kg AOAE; AOAE-H, lipopolysaccharide-challenged broilers fed a basal diet supplemented with 1000 mg/kg AOAE.
3) Each value is shown as mean ± SD (n = 6); Different superscript letters (a, b, and c) within the same row indicate significant difference between experimental groups (P <0.05).