FIGURE 5.

Glycocalyx remodeling at LD-APM contacts. (A) Representative 3D-projection images of mammary gland sections prepared from WT or Plin2-Null dams following removal of their litters for 2 h at L10 to inhibit milk secretion. The sections were immunostained with antibodies to BTN and XOR to identify LD-APM contacts and with Alexa633-tagged WGA (fl-WGA) to identify the glycocalyx. The images show exclusion of fl-WGA (blue green) from a BTN and XOR positive APM domain in WT sMEC, and partial exclusion of fl-WGA from a BTN and XOR positive APM domain in a Plin2 sMEC. Scale bars are 2 µm. (B,C) Pearson’s correlation coefficients describing the overlap between fl-WGA and BTN (B) or XOR (C) in 40–60 sMEC/animal from WT and Plin2-Null dams prepared as described in (A). Median values are indicated by white bars and average medians ±SEM are shown above each group. p-values for group differences were determined by nested t-test and are shown at the top of each figure. Pearson’s coefficients of 1, 0 and −1 represent perfect correlation (Corr), no correlation (Uncorr.) and perfect negative correlation (Anti-corr.) respectively as shown in each figure.