FIG. 7.

Effects of Synechococcus DnaK overproduction on E. coli cell morphology. E. coli MC4100 cells harboring Synechococcus dnaK expression plasmids were grown on LB agar plates in the presence or absence of 1 mM IPTG. After incubation for 18 and 36 h at 37°C, cells from multiple representative colonies were examined under a microscope (BX60; Olympus) with a U Plan F1 objective lens (×40), and photographs were taken with Neopan 400 Presto films (Fujifilm). In the absence of IPTG, the cells showed no difference in morphology after incubation for 18 and 36 h. As controls, MC4100 cells without plasmid were similarly grown on LB agar plates and examined after incubation for 18 and 36 h. Bar, 10 μm.