TABLE 1.

5′ oligonucleotide sequences of primers used to construct each dnaK expression plasmid^a

Plasmid	5′ oligonucleotide	Sequencea	Expressionc
pTrcDK1a	DK1TRC-Na	TAAGGAAAAAATTT (ATG)	±
pTrcDK1	DK1TRC-N	TAAGGAGGTAATTT (ATG)	+++
pTrcDK2	DK2TRC-N	TAAGGAACTGGACT (ATG)	+++
pTrcDK3a	DK3TRC-Na	TAAGGAAAGACAGC (ATG)	+
pTrcDK3	DK3TRC-N	TAAGGAGGTGACAGC (ATG)	+++
		TAAGGAGGTGATCb

^a Nucleotides that are identical to the complementary sequence of the 3′ end of E. coli 16S rRNA are highlighted in boldface. 

^b Complementary sequence of the 3′ end of E. coli 16S rRNA. 

^c Symbols indicate the expression levels of each DnaK protein in the range from detectable (±) to the maximum (+++) which we obtained in Fig. 6.