Table 2.

ANOVA report for the obtained results in CCD for different experimental runs of RLX/NRG NLCs.

	Factor 1	Factor 2	Factor 3	Response 1		Response 2		Response 3		Response 4
Run	Lipid (mg)	Surfactant (mg)	Sonication Time (mn)	Particle Size (nm)		PDI		EE%, RLX		EE%, NRG
				Actual	Predicted	Actual	Predicted	Actual	Predicted	Actual	Predicted
1	−1	0	0	131.6	131.56	0.238	0.2341	86.45	85.95	76.87	78.03
2	1	1	−1	159.38	159.82	0.291	0.2892	85.72	86.09	79.69	80.25
3	0	0	1	137.12	137.04	0.266	0.2691	91.05	90.77	85.07	85.05
4	0	0	0	143.2	140.73	0.261	0.2620	90.05	90.43	84.21	83.91
5	−1	1	−1	115.6	114.40	0.225	0.2297	84.22	83.88	78.03	77.22
6	0	0	0	141.05	140.73	0.261	0.2620	90.32	90.43	84.08	83.91
7	0	0	0	139.04	140.73	0.262	0.2620	90.16	90.43	84.42	83.91
8	1	0	0	166.03	167.64	0.282	0.2831	91.53	91.71	85.66	85.23
9	−1	1	1	121.7	122.98	0.224	0.2212	86.82	87.42	77.55	77.90
10	−1	−1	1	138.4	137.57	0.244	0.2466	84.72	84.43	78.81	78.07
11	0	0	2	142.15	140.73	0.261	0.2620	89.92	90.43	85.05	83.91
12	0	1	2	135.6	136.26	0.238	0.2369	88.63	88.45	81.01	81.13
13	1	−1	−1	169.02	167.34	0.317	0.3206	91.82	91.31	85.64	85.11
14	1	1	1	164.2	163.02	0.265	0.2662	85.83	85.38	79.73	79.51
15	0	−1	0	146.4	147.32	0.267	0.2653	89.72	89.57	83.03	83.64
16	0	0	0	142.15	140.73	0.259	0.2620	91.05	90.43	83.02	83.91
17	−1	−1	−1	134.44	135.22	0.268	0.2676	77.23	77.76	72.29	72.33
18	1	−1	1	163.5	164.31	0.289	0.2851	93.32	93.74	88.81	89.44
19	0	0	−1	132.6	134.26	0.297	0.2911	87.83	87.79	81.79	82.54
20	0	0	0	139.94	140.73	0.262	0.2620	90.45	90.43	84.15	83.91