Figure 3.

BcCps1 is involved in hyphal development 24 h after conidia germination. (A) Rupture and cell content leakage events (red arrows) in the hyphae of the mutant strain growing out of medium droplets on a plastic surface. Hyphae were stained with cotton blue. Scale bar: 50 nm—inlet: higher magnification. (B) Parental (B05.10) and mutant (ΔBccps1) hyphal development following conidia germination (after 24 h) on a rich solid medium (Agar) and inside a droplet of rich medium on a plastic surface (Plastic). Scale bar: 50 nm. (C) Expression kinetic of the Bccps1 gene (relative to the expression of three reference genes (BcactA, Bcef1α and Bcpda1) in the parental strain grown for 72 h in a rich liquid medium. Means and standard deviations were calculated based on three independent experiments (n = 9).