Table 3.
Primers for RT-PCR.
| Gene | Sequence (5′-3′) | Annealing Temperature |
Amplifier Size |
|---|---|---|---|
| MAP2/F | GCTAAATCGTAAGTGAGGGCTG | 60 °C | 241 bp |
| MAP2/R | TGGCTCTCTGGCTCTCTAGC | 60 °C | 241 bp |
| TUBB3/F | GGAGATCGTGCACATCCAGG | 62 °C | 385 bp |
| TUBB3/R | CAGGCAGTCGCAGTTTTCAC | 62 °C | 385 bp |
| NES/F | AACAGCGACGGAGGTCTCTA | 58 °C | 220 bp |
| NES/R | TTCTCTTGTCCCGCAGACTT | 58 °C | 220 bp |
| GFAP/F | CTCACCAAATTCCACCCGCA | 60 °C | 769 bp |
| GFAP/R | ACCGCACACAGTACCTGAAG | 60 °C | 769 bp |
| ACTB/F | CTGGGACGACATGGAGAAAA | 56 °C | 564 bp |
| ACTB/R | AAGGAAGGCTGGAAGAGTGC | 56 °C | 564 bp |
Note: primers obtained from Sigma-Aldrich®, St. Louis, MO, USA.