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. 2022 Sep 16;23(18):10822. doi: 10.3390/ijms231810822

Figure 6.

Figure 6

GW0742 reduces ER stress-induced astrocytic apoptosis induced by corticosterone. Representative TEM images are used to observe the structure of ER (a) in hippocampal astrocytes. Scale bar = 2 µm and 500 nm. The structure of the normal ER is shown as the green arrows. The structure of damaged ER is shown as the red arrows. GRP78 (b) and Chop (c) expressions are measured by western blot. Data is presented as means ± SEM. n ≥ 4, ** p < 0.01 vs. Con group; # p < 0.05; ## p < 0.01 vs. CORT group; †† p < 0.01 vs. CORT + GW0742 group, by one-way ANOVA and Tukey’s test. (d,e) Knocking down UCP2 turned over the protein expressions of GRP78 and Chop in astrocytes treated with corticosterone and GW0742. Data is presented as means ± SEM. n ≥ 3, * p < 0.05 vs. Con group in the Ctrl; # p < 0.05 vs. CORT group in the Ctrl; † p < 0.05 vs. CORT + GW0742 group in the Ctrl, by two-way ANOVA and Tukey’s test. (f,g) Quantitative analysis of the apoptosis rate in astrocytes. Data is presented as means ± SEM. n = 5, *** p < 0.001 vs. Con group; ### p < 0.001 vs. CORT group; ††† p < 0.001 vs. CORT + GW0742 group, by one-way ANOVA and Tukey’s test. (h–o) Western blotting is used to analyze the expression of p-IRE1α/IRE1α, p-PERK/PERK, AFT6, p-JUK/JUK, C-Caspase3/Caspase3 and C-Caspase12/Caspase12 among the different group. Data is presented as means ± SEM. n ≥ 4, ** p < 0.01, *** p < 0.001 vs. Con group; ## p < 0.01 vs. CORT group; † p < 0.05, †† p < 0.01 vs. CORT + GW0742 group, by one-way ANOVA and Tukey’s test.