FIG. 1.

Physical map and genetic organization of Streptomyces plasmid pIJ101. The highlighted region includes previously determined genetic functions related to transmission of the plasmid (12, 13, 15), which are shown alongside their respective ORFs (filled arrows) or, in the case of clt, its locus (filled box). The remaining plasmid region includes functions involved in replication of the plasmid (2, 6, 13), and these are also indicated beside their respective ORFs (striped arrows) or loci (striped boxes). Small ORFs (i.e., orf56, orf66, orf79, and orf85) (11) whose functions remain undetermined are indicated in both regions. The sequence that is deleted (Δ) in plasmid pIJ303ΔkilB, which is a derivative of the conjugative, thiostrepton-resistant pIJ101 plasmid pIJ303 (13), is indicated.