FIG. 4.

Western blot analysis of KilB protein in S. lividans submerged cultures. Spores of TK23 (A), either containing or lacking pIJ303, or of strain NWR2 (B) were used to inoculate yeast extract-malt extract medium (including thiostrepton for NWR2), and at successive time points (indicated numerically) during exponential (E) and stationary (S) growth, cells were harvested. Preparation of extracts and their analysis by SDS-PAGE and Western blotting for KilB protein were performed as indicated in the legend for Fig. 2. Only one time point during stationary growth for strain TK23 is shown, although multiple time points in both exponential and stationary growth of this strain were analyzed (data not shown).