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Phosphopantetheination of acyl carrier protein detected as free thiol. The graph shows the amount of phosphopantetheinatation of the acyl acrrier protein (ACP), the thiolation domain for lipid synthesis. The amount of phosphopantetheination is given as the resultant micromolar amount of CoA added to the 15 µM of starting ACP protein. Error bars show the standard deviation of triplicate reactions for the Clade one, two and three PPTases colored blue, red and green, respectively.
