Table 2.
Primers used in this study.
| Primer Name | Sequence 5′ to 3′ | Length | Annealing Temp. °C |
|---|---|---|---|
| PPT1_CDSF4 § | GCTTACAGTGGAGGCCCTACTTCCAATGGG | 30 | 74.9 |
| PPT2_CDSF4 § | TCCCTGCGGTGTCCAACTTCAAGCTTTACA | 30 | 72.1 |
| DTR2 § | CATCTTGCTAGCTCGCGATCTTGAAGTAGTC | 31 | 72.1 |
| Dino_SL § | TCCGTAGCCATTTTGGCTCTAA | 21 | 59.5 |
| anchoredDTR2 ☥ | CATCTTGCTAGCTCGCGATCTTGAAGTAGTCTTTTTTTTTTTTTTTTTTTTS | 52 | 41.9 * |
Primers listed with an “§” were used in PCR amplification, while those with an “☥” were use in reverse transcription. The “anchored DTR2” annealing temperature denoted with a “*” is for the poly-T region only.