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Proposed model to explain blockage of secretion for hybrids containing disulfide bonds. (A) The hybrid carrying a preformed disulfide bond approaches the channel entrance, is recognized by the secretion machinery (PrtD), but cannot go across the channel. (B) The hybrid is stacked inside the channel during secretion. Disulfide bonds may be formed at this stage. (C) The hybrid has formed inclusion bodies by intermolecular S—S bonds and cannot reach the channel or enter it.
