TABLE 2.
List of plasmids used in this work
| Plasmid(s)a | Relevant properties | Source or reference |
|---|---|---|
| pSE280 (Apr), pSE420 (Apr) | Cloning and expression vectors | Invitrogen |
| pGEM-T, pGEMT Easy (Knr) | Vector for cloning of PCR fragments | Promega Corp. |
| pCR2.1 (Apr) | Vector for cloning of PCR fragments | Invitrogen |
| pRUW500 (Apr) | prtB (E. chrysanthemi protease B gene) | 20 |
| pRUW4 (Cmr) | prtD, prtE, and prtF genes | 20 |
| pACYC184 | Low-copy-number cloning vector | New England BioLabs |
| pKK233-2 (Apr) | IPTG-inducible expression vector | Pharmacia Biotech |
| pACYC-dsbC | E. chrysanthemi dsbC gene in pACYC184 | This work |
| pGFP | GFP gene in pUC19 | Clontech |
| pGFP-181 | GFP-protease B long hybrid gene in pSE420 | This work |
| pSE280-93CTPB | Short C-terminal protease B gene in pSE280 | This work |
| pSE420-93CTPB | Short C-terminal protease B gene in pSE420 | This work |
| pSE420-181CTPB | Long C-terminal protease B in pSE420 (Eco47III-HindIII) | This work |
| pSE420-181CTPB2 | Long C-terminal protease B in pSE420 (SmaI-HindIII) | This work |
| pECH42 | T. harzianum endochitinase cDNA | M. Gidekel |
| pCHIT-9, pCHIT-1 | Endochitinase gene in pSE420 | This work |
| pCHIT-93CTPB | Endochitinase-protease B short hybrid in pSE420 | This work |
| pCHIT-181CTPB | Endochitinase-protease B long hybrid in pSE420 | This work |
| pERY727 | Synthetic hEPO gene in pSVK10 | A. Venegas |
| phEPO-93CTPB | hEPO-protease B short hybrid gene in pSE420 | This work |
| phEPO-181CTPB | hEPO-protease B long hybrid gene in pSE420 | This work |
| pOmpC-181CTPB | S. typhi OmpC (205 amino acids) in pSE420-181CTPB-2 | This work |
| pOmpC-Bgl2 | A BglII insert of the S. typhi ompC gene in pUC19 | 69 |
| pβGAL-181CTPB | Initial codons of β-galactosidase plus polylinker-CTPB in pCR2.1 | This work |
| pKKTGH-23 | tGH cDNA in pKK233-2 | 49 |
| ptGH-93CTPB | tGH-protease B short hybrid gene in pSE420 | This work |
| ptGH-181CTPB | tGH-protease B long hybrid in pSE420 | This work |
a
Preparative amounts of plasmids were obtained by using QIAGEN columns according to vendor instructions.