Figure 3.

SH upregulated expression and plasma membrane localization of NIS and increased RAI uptake in BCPAP and TPC-1 cells. (A,B) Western blot analysis shows the expression of NIS in whole cell lysates (A), cell membrane (B), and cytoplasmic proteins (B) after 48 h with SH treatment. GAPDH and ATP were used as the loading control. (C) Quantitative analysis of band intensities on Western blots. (D) Immunofluorescence of NIS protein in BCPAP and TPC-1 cells treated with 4 mM SH for 48 h was detected using confocal microscope with high magnification fields (63×). Scale bar represents 25 μm. (E) BCPAP and TPC-1 cells were treated with fixed concentrations of SH (0, 2, 4, 6 mM) for 48 h, followed by ¹²⁵I uptake assays. In addition, KClO4 was used as a competitive inhibitor of iodide transport. The cells were preincubated with 300 μM KClO4 for 30 min to inhibit ¹²⁵I uptake, followed by the ¹²⁵I uptake test. Data are presented as mean ± SD of values. * p < 0.05 vs. control. ** p < 0.005 vs. control. ^# p < 0.05 vs. relevant group treated by SH only. RAIU: RAI uptake.