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. 2022 Sep 3;10(9):1780. doi: 10.3390/microorganisms10091780

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of oral microbial biofilms grown in SHI medium with CaCl₂ supplementation evaluated at day 1 (D1), day 3 (D3), and day 5 (D5). (A) Biofilm metabolic activity: The metabolic activity of the biofilms was assessed by measuring formazan formation from MTT at the optical density of 570 nm. ** Represents p ≤ 0.01, and **** represents p ≤ 0.0001 (B) Extracellular DNA (eDNA) composition: eDNA is a ubiquitous and pivotal component of biofilm. Denaturing gradient gel electrophoresis (DGGE) profiles of eDNA of oral microbial biofilms grown in 1. SHI medium, 2. SHI medium + 1 mM CaCl₂, and 3. SHI medium + 5 mM CaCl₂ at day 1 (D1), day 3 (D3), and day 5 (D5).