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. 2022 Aug 27;10(9):1405. doi: 10.3390/vaccines10091405

Figure 3.

Figure 3

ELISpot analysis of stimulated T cells. Splenocytes collected after booster vaccination were added into chicken interferon-γ (ChIFN-γ)-coated wells and stimulated with soluble antigen for 32.5 h. The results represent the number of ChIFN-γ-secreting cells. PBS—non-vaccinated group; Oral/IM—prime-boost vaccinated group with the candidate strain via oral–intramuscular route; and IM/Oral—prime-boost vaccination with the candidate via intramuscular–oral route. * p < 0.05.