Figure 3.
ELISpot analysis of stimulated T cells. Splenocytes collected after booster vaccination were added into chicken interferon-γ (ChIFN-γ)-coated wells and stimulated with soluble antigen for 32.5 h. The results represent the number of ChIFN-γ-secreting cells. PBS—non-vaccinated group; Oral/IM—prime-boost vaccinated group with the candidate strain via oral–intramuscular route; and IM/Oral—prime-boost vaccination with the candidate via intramuscular–oral route. * p < 0.05.