FIG. 3.

(A) Determination of the tsp of smcR. Fifty micrograms of total RNA extracted from YJ016 was used in the primer extension reaction. Nucleotide sequences (GATC) of the noncoding strand up- and downstream of tsp obtained with the same oligoprimer are indicated. A G (indicated by an arrow) 87 bp upstream of the initiation codon was identified as the tsp. (B) Sequence alignment of the promoter regions of smcR, luxR, opaR, and hapR. The underlines indicate the promoter sequences (−10 and −35) and the asterisk denotes the tsp.