FIG. 5.

(A) Restriction map of smcR and the flanking regions. The arrow indicates the direction of transcription. The extent of deletion (blank bar) in vvpR and the probe used in Southern hybridization are also depicted. (B) Detection of the deletion in smcR in the chromosome of V. vulnificus. Ten micrograms of genomic DNA was digested by EcoRV and then subjected to electrophoresis on a 1.2% agarose gel. Ma, molecular weight standards; W, V. vulnificus YJ016 (wild type); I, CP154 (YJ016 with pVR19 integrated in the chromosome); M, CP156 (ΔsmcR).