TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| V. vulnificus | ||
| YJ016 | Clinical isolate | Laboratory collection |
| YJ024 | Translucent mutant of YJ016 | 9 |
| CP080 | YJ016 Δvvp | 30 |
| CP154 | YJ016 smcR::pVR19 | This study |
| CP156 | YJ016 ΔsmcR | This study |
| CP194 | CP156/pVR21 | This study |
| E. coli | ||
| DH5α | supE44 lacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 7 |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir Kmr Nalr | 16 |
| LLM1956 | DH5α/pRS205 | 14 |
| Plasmids | ||
| pRS205 | pUC19 with the lux operon of V. harveyi | 14 |
| pJRD215 | Shuttle vector; Kmr | 5 |
| pUC19 | Cloning vector; Apr | Laboratory collection |
| pCVD442 | Suicide vector; Apr | 6 |
| pVR11 | pBR322 inserted with a 3.3-kb fragment containing the intact smcR gene | This study |
| pVR18 | pVR11 with a 445-bp deletion in smcR (ΔsmcR) | This study |
| pVR19 | pCVD442 with the NheI-NheI fragment that contains ΔsmcR cloned from pVR18 | This study |
| pVR21 | pJRD215 with the NheI-NheI fragment that contains the intact smcR cloned from pVR11 | This study |
| pVR22 | pJRD215 with the NheI-NheI fragment that contains ΔsmcR cloned from pVR18 | This study |
a
Abbreviations: Apr, Kmr, and Nalr are resistance to ampicillin, kanamycin, and nalidixic acid, respectively.