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. 2022 Sep 16;23(18):10816. doi: 10.3390/ijms231810816

Figure 2.

Figure 2

Anxiety-like behavior. (A,B): Open field (OF) test. (A): There was a significant difference in time spent in immobile posture: 3xTg-AD mice spent significantly less time with active movement and more with immobility compared to control both in 4- (* p = 0.02) and in 8-month (* p = 0.04) groups. (B): 3xTg-AD animals in both age groups spent more time with self-grooming than the controls, however, the difference was only marginally significant ((*) p = 0.09). For 8-month group, this difference was further reduced (p = 0.24). (C): Elevated plus maze (EPM) test. There was no significant difference between genotypes either in the classical parameters (open arm time, data not shown), or in locomotion-independent anxiety parameter, the % of open arm frequency. (D–F): Light-dark (LD) test. (D): Significant difference was found between genotypes in percentage of time spent in the dark compartment of the box (genotype: * p = 0.00). (E): The frequency of stepping into the dark compartment was also significantly lower in the 3xTg-AD mice compared to the controls (genotype: * p = 0.00). (F): There was also a significant difference in the latency of stepping into the dark compartment in case of the 8-month-old animals, 3xTg-AD mice entered later than the controls (age*genotype: * p = 0.04; 8-month-old post-hoc: # p = 0.01). (G–H): Novelty suppressed feeding (NSF) test. (G): There was a significant difference in the time spent with immobility between genotypes in both age groups (genotype: * p = 0.03). More precisely, 4-month-old 3xTg-AD mice spent more time with this behavior than their controls, which suggested enhanced anxiety-like behavior. (H): 3xTg-AD mice spent more time with eating without age difference (genotype: * p = 0.00). (I): Social interaction (SI) test. There was a significant difference between genotypes in the time spent with social behavior in case of the 8-month-old animals with age difference (age*genotype: * p = 0.05; 8-month-old post-hoc: # p = 0.01). The 3xTg-AD mice spent less time with friendly social behavior compared to the controls.