Table 2.
Gene | Primer | Sequences (5′-3′) |
---|---|---|
IL-1β | Forward | GCAACTGTTCCTGAACTCAACT |
Reverse | ATCTTTTGGGGTCCGTCAACT | |
IL-6 | Forward | TAGTCCTTCCTACCCCAATTTCC |
Reverse | TTGGTCCTTAGCCACTCCTTC | |
INF-γ | Forward | TCAAGTGGCATAGATGTGGAAGAA |
Reverse | TGGCTCTGCAGGATTTTCATG | |
IL-4 | Forward | ACAGGAGAAGGGACGCCAT |
Reverse | GAAGCCCTACAGACGAGCTCA | |
T-bet | Forward | AGCAAGGACGGCGAATGTT |
Reverse | GTGGACATATAAGCGGTTCCC | |
GATA-3 | Forward | AAGCTCAGTATCCGCTGACG |
Reverse | GTTTCCGTAGTAGGACGGGAC | |
STAT1 | Forward | TCACAGTGGTTCGAGCTTCAG |
Reverse | CGAGACATCATAGGCAGCGTG | |
STAT6 | Forward | CTCTGTGGGGCCTAATTTCCA |
Reverse | CATCTGAACCGACCAGGAACT | |
GAPDH | Forward | ACCTGCCAAGTATGATGACATCA |
Reverse | GGTCCTCAGTGTAGCCCAAGAT |