Figure 1.

Overview of LTX-315 anticancer peptide and experimental strategy: (A) amino acid structures, sequence, and 3D molecular model of LTX-315 peptide. Hydrophobic (Trp and Dip) and cationic (Lys) amino acids are depicted in yellow and blue, respectively; (B) proposed biological mechanism of how LTX-315 peptide exhibits anticancer activity based on cancer cell membrane disruption; and (C) experimental strategy to track membrane-peptide interactions using the supported lipid bilayer (low curvature), intact vesicle (high curvature), and tethered bilayer lipid membrane platforms with different membrane surface charges. Measurements were conducted using the quartz crystal microbalance-dissipation (QCM-D) and electrochemical impedance spectroscopy (EIS) techniques.