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. 2022 Sep 12;23(18):10558. doi: 10.3390/ijms231810558

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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EIS tracking of LTX-315 peptide interactions with tethered lipid bilayers depending on membrane surface charge: (A) time-dependent changes in conductance (G_m) and capacitance (C_m) signals upon LTX-315 peptide addition to a 70/30 DOPC/DOPS tBLM platform. LTX-315 peptide was added to the tBLM platform starting at t = 10 min (arrow i), followed by a buffer washing step from t = 30 min onward (arrow ii); corresponding EIS data for (B) 85/15 DOPC/DOPS, (C) 100 DOPC, (D) 85/15 DOPC/DOEPC, and (E) 70/30 DOPC/DOEPC tBLM platform; and (F) summary of G_m and C_m shifts upon LTX-315 peptide addition (treatment) and after buffer washing (post-wash) for tBLMs with different membrane surface charges. The data are reported as the mean ± standard deviation from n = 3 measurements.