Figure 5.

Polar auxin transport in roots of dig5 and wild type seedlings. (A) Basipetal auxin transport. (B) Acropetal auxin transport. 100 nM [³H]-IAA with or without NPA (100 µM) was applied either to the root tip (A) or shoot-root junction (B) of seedlings on agar plates followed by incubation in the dark for 5 h (A) or 16 h (B). The distal segments of the roots were harvested to measure the radioactivity. Data were means and standard errors of 3 replicates, each with at least 10 seedlings.