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. 2022 Sep 13;23(18):10635. doi: 10.3390/ijms231810635

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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ROC analysis—the combination of 3 basic clinical characteristics (maternal age and BMI values at early stages of gestation and an infertility treatment by assisted reproductive technology) and 8 dysregulated microRNA biomarkers (miR-1-3p, miR-20a-5p, miR-20b-5p, miR-100-5p, miR-125b-5p, miR-195-5p, miR-499a-5p, and miR-574-3p). At a 10.0% FPR, 50.50% pregnancies destinated to develop GDM managed by diet only were identified during the first trimester of gestation. This represents 51 out of 101 pregnancies correctly predicted to develop GDM and 8 out of 80 normal pregnancies predicted false positively to develop GDM.