Table 3.
MicroRNA expression profiles in peripheral blood leukocytes in early stages of gestation in pregnancies destinated to develop GDM and normal term pregnancies.
| Mann-Whitney Test Results GDM Overall (n = 121) vs. Normal Term Pregnancies (n = 80) |
|||
|---|---|---|---|
| Median (IQR) | Mean (SD) | p-Value | |
| miR-1-3p | 0.135 (0.071–0.254) vs. 0.075 (0.033–0.198) | 0.259 (0.525) vs. 0.176 (0.303) | p = 0.0028 ** |
| miR-16-5p | 1.216 (0.968–1.725) vs. 1.411 (0.890–1.980) | 1.495 (0.981) vs. 1.646 (1.129) | p = 0.5781 |
| miR-17-5p | 1.527 (1.181–2.311) vs. 1.384 (0.971–1.923) | 1.973 (1.473) vs. 1.748 (1.312) | p = 0.0538 |
| miR-20a-5p | 2.215 (1.493–3.398) vs. 1.576 (0.991–2.413) | 3.037 (3.068) vs. 1.909 (1.370) | p < 0.001 *** |
| miR-20b-5p | 2.662 (1.812–3.959) vs. 1.976 (1.111–2.675) | 3.706 (3.878) vs. 2.377 (2.291) | p < 0.001 *** |
| miR-21-5p | 0.344 (0.231–0.460) vs. 0.320 (0.167–0.538) | 0.433 (0.420) vs. 0.394 (0.219) | p = 0.2418 |
| miR-23a-3p | 0.239 (0.168–0.436) vs. 0.185 (0.103–0.376) | 0.367 (0.337) vs. 0.296 (0.329) | p = 0.0065 * |
| miR-24-3p | 0.292 (0.228–0.372) vs. 0.326 (0.196–0.468) | 0.331 (0.197) vs. 0.384 (0.284) | p = 0.5730 |
| miR-26a-5p | 0.699 (0.500–0.926) vs. 0.633 (0.410–1.066) | 0.837 (0.670) vs. 0.776 (0.521) | p = 0.3022 |
| miR-29a-3p | 0.405 (0.282–0.575) vs. 0.372 (0.221–0.545) | 0.510 (0.396) vs. 0.407 (0.245) | p = 0.0840 |
| miR-92a-3p | 2.179 (1.604–3.084) vs. 2.327 (1.188–3.743) | 2.702 (2.226) vs. 2.807 (2.132) | p = 0.9812 |
| miR-100-5p | 0.0023 (0.0013–0.0036) vs. 0.0013 (0.0006–0.0027) | 0.0030 (0.0039) vs. 0.0018 (0.0016) | p < 0.001 *** |
| miR-103a-3p | 1.565 (0.963–2.541) vs. 1.203 (0.815–2.425) | 2.121 (2.252) vs. 1.770 (1.466) | p = 0.1547 |
| miR-125b-5p | 0.0041 (0.0025–0.0057) vs. 0.0030 (0.0016–0.0054) | 0.0049 (0.0046) vs. 0.0036 (0.0027) | p = 0.0034 ** |
| miR-126-3p | 0.328 (0.231–0.509) vs. 0.272 (0.140–0.432) | 0.462 (0.551) vs. 0.336 (0.270) | p = 0.0137 * |
| miR-130b-3p | 0.745 (0.476–1.409) vs. 0.702 (0.407–1.157) | 1.075 (0.960) vs. 1.163 (2.425) | p = 0.2105 |
| miR-133a-3p | 0.109 (0.061–0.220) vs. 0.110 (0.550–0.233) | 0.193 (0.265) vs. 0.232 (0.483) | p = 0.8750 |
| miR-143-3p | 0.048 (0.030–0.880) vs. 0.038 (0.016–0.089) | 0.073 (0.086) vs. 0.058 (0.057) | p = 0.0260 |
| miR-145-5p | 0.176 (0.125–0.236) vs. 0.161 (0.980–0.243) | 0.209 (0.153) vs. 0.195 (0.143) | p = 0.2025 |
| miR-146a-5p | 1.224 (0.821–1.843) vs. 1.225 (0.578–1.765) | 1.658 (1.541) vs. 1.388 (1.096) | p = 0.1415 |
| miR-155-5p | 0.619 (0.434–0.778) vs. 0.607 (0.361–1.614) | 0.703 (0.523) vs 1.247 (1.439) | p = 0.2987 |
| miR-181a-5p | 0.250 (0.175–0.379) vs 0.181 (0.141–0.330) | 0.330 (0.318) vs 0.246 (0.184) | p = 0.0065 * |
| miR-195-5p | 0.267 (0.168–0.487) vs 0.106 (0.048–0.271) | 0.470 (0.690) vs 0.227 (0.364) | p < 0.001 *** |
| miR-199a-5p | 0.080 (0.037–0.159) vs 0.058 (0.023–0.111) | 0.136 (0.223) vs 0.096 (0.131) | p = 0.0288 |
| miR-210-3p | 0.102 (0.074–0.154) vs 0.138 (0.075–0.224) | 0.134 (0.105) vs 0.186 (0.180) | p = 0.0952 |
| miR-221-3p | 0.644 (0.448–0.969) vs 0.548 (0.293–0.906) | 0.815 (0.736) vs 0.693 (0.561) | p = 0.0947 |
| miR-342-3p | 3.069 (2.122–4.110) vs 2.542 (1.551–4.206) | 3.605 (2.724) vs 3.307 (2.383) | p = 0.1947 |
| miR-499a-5p | 0.460 (0.231–0.780) vs 0.269 (0.089–0.587) | 0.758 (1.070) vs 0.477 (0.566) | p < 0.001 *** |
| miR-574-3p | 0.275 (0.180–0.395) vs 0.181 (0.117–0.292) | 0.354 (0.332) vs 0.222 (0.156) | p < 0.001 *** |
MicroRNA gene expression is compared between groups using the Mann–Whitney test. Statistically significant results are marked in bold. Median (interquartile range, IQR) and mean (standard deviation, SD) fold values of relative gene expression of samples (2−∆∆Ct) are presented. Statistical significant data after Benjamini–Hochberg correction are marked by * for α = 0.05, ** for α = 0.01, and *** for α = 0.001.