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. 2022 Sep 10;12(18):3138. doi: 10.3390/nano12183138

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Bio-MnO₂ NPs enhanced radiotherapy (RT) in vitro. (a) Cell viabilities of A549, PC9 and H520 cells incubated with Bio-MnO₂ NPs at various concentrations with or without X-ray radiation. (b) Colony formation assay of NSCLC cells after different treatments and statistical graphs of colony formation assay. (c,d) Representative images and statistical graphs of Ki-67 immunofluorescence. Scale bar, 100 μm. (e,f) Cell apoptosis assay via flow cytometry. (g) Immunoblotting of cell apoptosis related proteins. *, p < 0.05; **, p < 0.01; ***, p < 0.001.