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. 2022 Sep 10;12(18):3138. doi: 10.3390/nano12183138

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Bio-MnO₂ NPs plus radiation increased DNA damage in NSCLC cells. (a) Representative images of double-stranded DNA (dsDNA) immunofluorescent staining (red) after different treatments. Scale bar, 50 μm. (b) Representative images of γ-H2AX immunofluorescence (green) after different treatments. Scale bar, 50 μm. (c) Statistical graphs of dsDNA and γ-H2AX immunofluorescence. (d) Representative images of comet assay after different treatments in NSCLC cells. (e) Statistical graphs of comet assay. (f) Immunoblotting of DNA damage and repair related proteins. **, p < 0.01; ***, p < 0.001. ****, p < 0.0001.