Figure 3.

MRE/rprORF causes apoptosis in C6/36 cells. (A) C6/36 cells were infected with MRE/rprORF, MRE/control, or 5′dsMRE16ic (labeled MRE16). After 48 h, the cells were collected, DNA was extracted, and run on an agarose gel containing ethidium bromide. (B) Cells infected with MRE/rprORF, MRE/rpr, or MRE/control were collected and lysed at 24 and 48 hpi. Caspase activity from these cell lysates was measured by examining their ability to cleave the fluorogenic substrate Ac-DEVD-AFC. Fluorescence measurements were taken every 15 min for 1 h. Three biological replicates were performed. The data were plotted using simple linear regression. Error bars indicate SEM.