Table 2.

Mutations detected at >1% frequency in MRE/rprORF stock virus and infected mosquito carcasses.

Mutation	Aa Change 1	S1 2	S2	C6	C9	C11	C17	C18	C19	C20	C24	C36	C37
C8807T	none	0.0265 3	0.0249	0.0242	0.0243	0.0265	0.0268	0.0256	0.0257	0.0248	0.0222	0.0219	0.0259
T8826C	L73P (ubi)							0.2832
C8844T	A4V (rpr)								0.7778
C8937T	L40P (rpr)						0.9950					0.0143	0.9946
C8943T	T37I (rpr)	0.0135	0.0143	0.0132	0.0140	0.0143	0.0139	0.0138	0.0142	0.0142	0.0142	0.0147	0.0138

¹ Predicted amino acid changes caused by point mutations in ubiquitin (ubi) or Reaper (rpr). Numbers represent the affected amino acid position in the protein and letters represent the predicted change. For example, L73P was a change from leucine to proline at position 73 of ubiquitin. ² Samples S1 and S2 were independent RNA extractions from MRE/rprORF stock virus, while samples C6–C37 were MRE/rprORF-infected carcasses. ³ Frequency of mutation observed in sample. The minimum average read depth in any of the samples was 79 K. Blank spaces indicate that the mutation was present at <1%.